Chemistry: analytical and immunological testing – Hemoglobin – myoglobin – or occult blood
Reexamination Certificate
1999-05-26
2001-11-13
Wallenhorst, Maureen M. (Department: 1743)
Chemistry: analytical and immunological testing
Hemoglobin, myoglobin, or occult blood
C436S164000, C422S051000
Reexamination Certificate
active
06316263
ABSTRACT:
BACKGROUND OF THE INVENTION
I. Field of the Invention
The present invention relates to a method for measuring antioxidant activities of samples and method for diagnosing diabetes or hyperlipidemia using the same.
II. Description of the Related Art
Methods for measuring antioxidant activities of samples, that is, the activities to prevent oxidation of other substances, are known. For example, WO92/10759 discloses a method for measuring antioxidant activity of blood plasma for the purpose of diagnosing myocardial infarction or for diagnosing conditions of a patient for the likelihood of onset of myocardial infarction. In this method, blood plasma which is a test sample, metmyoglobin, hydrogen peroxide, a coloring agent such as 2,2′-azinobis(3-ethylbenzthiazoline 6-sulfonate) (hereinafter referred to as “ABTS”) are reacted and the absorbance after the reaction is measured. With this method, if the antioxidant activity of blood plasma is high, oxidation of metmyoglobin by hydrogen peroxide is inhibited by the blood plasma, so that coloring is inhibited. On the other hand, if the antioxidant activity of the blood plasma is low, oxidation of metmyoglobin by hydrogen peroxide is not inhibited very much, so that coloring is not inhibited very much accordingly. Therefore, by measuring the absorbance of the reaction mixture after the reaction, the antioxidant activity of the blood plasma can be measured.
Needless to say, it is advantageous to provide a method for measuring antioxidant activities of test samples, whose sensitivity is higher than that of the above-mentioned conventional method.
SUMMARY OF THE INVENTION
Accordingly, an object of the present invention is to provide a method for measuring antioxidant activities of test samples, whose sensitivity is higher than that of the conventional method.
The present inventors intensively studied to discover that by using methemoglobin in place of metmyoglobin used in the conventional method, the sensitivity of the measurement is increased by not less than three times, thereby completing the present invention.
That is, the present invention provides a method for measuring antioxidant activity of a test sample comprising the steps of reacting methemoglobin, an oxidizing agent which oxidizes methemoglobin, a coloring agent which generates color by reaction with methemoglobin in the presence of said oxidizing agent, and a test sample whose antioxidant activity is to be measured; and measuring the generated color. The present invention also provides a method for diagnosing diabetes or hyperlipidemia comprising carrying out the above-described method of the present invention using a body fluid such as blood plasma or serum as the test sample.
By the present invention, a method for measuring antioxidant activities of test samples, whose sensitivity of measuring antioxidant activities of test samples is higher than that of the conventional method, was provided. Further, by the present invention, a highly sensitive diagnostic method for diabetes or hyperlipidemia was provided.
REFERENCES:
patent: 4278439 (1981-07-01), White
patent: 5620863 (1997-04-01), Tomasco et al.
patent: 5753452 (1998-05-01), Smith
patent: 5773301 (1998-06-01), Ziegler
patent: 5843691 (1998-12-01), Douglas et al.
patent: 5858794 (1999-01-01), Malin
patent: 5885789 (1999-03-01), Kardos et al.
patent: WO 9119979 (1991-12-01), None
patent: WO 9210759 (1992-06-01), None
Nagase et al., Serum effects, Guanidini Compounds, 2nd, Ed., pp. 317-324, 1997.*
Lissi et al., Photochemistry and Photobiology, vol. 60, No. 5, pp 405-411, 1994.*
Nakano et al., Simple Luminescence Method, Biochem and Biophys. Res. Comm., pp 940-946, Jul. 1994.*
Murray et al., Critical Care Medicine, vol. 15, No. 8, pp 797-98, 1987.*
Journal of Environmental Science and Health, A20(1), 21-35 (1985), John Kemp et al.
Chem. abstr., vol. 123, No. 5, Jul. 31, 1995 (Columbus, OH, USA), p. 490, col. 2.
Miller, N.J. et al. “Total antioxidant activity of low density lipoproteins and the relationship with alpha-tocopherol status”, FEBS Lett. 1995, 365(2,3), 164-6 (Eng).
Jaworowski, A. et al. “Calponin reduces shortening velocity in skinned taenia coli smooth muscle fibres”, FEBS Lett. 1995, 365, 167-171 (Sweden).
Itakura Hiroshige
Kimura Masahiro
Kondo Kazuo
Yamamoto Hiroo
Birch & Stewart Kolasch & Birch, LLP
TFB, Inc.
Wallenhorst Maureen M.
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