Method for measuring antigens or antibodies in biological fluids

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic

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422 63, 422 64, 422 67, 422 8205, 422 8209, 422 681, 435 79, 435 792, 435 794, 4352871, 4352872, 4352887, 436 43, 436 47, 436 49, 436 50, 436165, 436518, 436524, 436536, 436538, 436541, 436807, 436809, G01N 33553, G01N 3502

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056984502

ABSTRACT:
The invention relates to a method of measuring antigens or antibodies in biological fluids in appropriately designed reaction cells in an automated analytical apparatus. The method includes the steps of contacting, in the reaction cell, the biological fluid with antibodies specific for a desired analyte antigen, which antibodies are coated on magnetic particulate carrier, under conditions such that binding of the antibody to the desired analyte antigen occurs, and detecting the presence or absence of an immunocomplex formed between the antibody and the desired analyte antigen. The automated analytical apparatus includes a closed circuit transfer path having means for transferring cells around the entire transfer path, and a thermostating period for each analysis to be performed with the automated analytical apparatus. The transfer path includes a loading station, a reagent delivery station, a mixing and washing station, a separating station and a discharging station. A specific code is placed on each cell for identifying a kind of analysis to be effected with respect to that cell.

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WO, A 83/00393 (American Hospital Supply Copr.) 3 Feb. 1983.
WO A, 83/01119 (Commonwealth Serum Laboratorie Commission) 31 Mar. 1983.

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