Method for measuring anti-inflammatory properties of a compositi

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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73 641, 356442, 436 63, 436164, 436183, C12Q 102, G01N 2159, G01N 3348

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active

046056181

ABSTRACT:
Disclosed is a method for measuring aggregation in sponge cells using a clinical aggregometer. In addition, using the marine sponge cell as an in vitro model for neutrophil aggregation, a method to determine the relative anti-inflammatory properties of non-steroidal anti-inflammatory pharmaceutical agents by clinical aggregometry is also disclosed.

REFERENCES:
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patent: 4252536 (1981-02-01), Kishimoto et al.
patent: 4329152 (1982-05-01), Lauwerys et al.
Rice et al., "Two Ca.sup.2+ Functions are Demonstrated by the Substitution of Specific Divalent and Lanthanide Cations for the Co.sup.2+ Required by the Aggregation Factor Complex from the Marine Sponge, Microclone Prolifera" The Journal of Biological Chemistry 258(10), 6394-9, 1983.
Rich, Abby M. et al., "Calcium Dependent Aggregation of Marine Sponge Cells is Provoked by Leukotriene B.sub.4 and Inhibited by Inhibitors of Arachidonic Acid Oxidation," 1984, Biochem. Biophys. Res. Commun., 121(3), 863-70.
Misevic, et al., "Cell Binding Fragments from a Sponge Proteoglycen-Like Aggregation Factor," The Journal of Biological Chemistry 257(12), 6931-6, 1982.

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