Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-03-30
1997-03-11
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 94, 435968, 250361R, 536 243, C12Q 168, C12P 1924, C07H 2104, G01T 120
Patent
active
056100167
ABSTRACT:
The invention provides an assay by which detection and/or quantitation of adenyl group-containing substances can be performed simply, with high sensitivity and high signal-to-noise ratio. In particular, the method provides an assay in which the chemiluminescence of a chemiluminescent substance, made by reacting a glyoxal derivative with an adenyl group in the substance to be detected/quantitated in the presence of a heteropolyacid or a heteropolyacid salt, is measured.
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Abstract of Lukovskaya, N. et al., "Chemiluminescence reactions with luminol . . . ", Chem. Abstracts vol. 88, p. 609, abstr. No. 83093y, 1978.
Chan-Curtis et al, (1970), "Chemical studies on an acriflavine-phosphotungstate complex; staining of phage deoxyribonucleic acid molecules for the electron microscope", J. Histochem. Cytochem. 18(9):628-634.
Peats et al, (1983), "A sensitive procedure for silver staining proteins in agarose gels", Biotechniques 1(3):154-156.
Kuroda et al, 41st Annual Meeting of The Japan Society of Analytical Chemistry, p. 472 (Aug. 27, 1992).
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Sato Naofumi
Shirakawa Kamon
Fredman Jeffrey
Mochida Pharmaceutical Co. Ltd.
Sisson Bradley L.
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