Method for measuring a concentration of saccharides having a low

Details Method for measuring a concentration of saccharides having a low Method for measuring a concentration of saccharides having a low

1986-02-04

1989-09-12

Nucker, Christine M.

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

422 68, 422 69, 435288, 435299, 435311, 436 95, 436178, G01N 3096

Patent

active

048659959

ABSTRACT:
A method for measuring the concentration of low molecular weight saccharides in a liquid by placing the liquid in contact with one side of a membrane which allows saccharides to pass through, but not polysaccharides. A detecting liquid containing polysaccharides is placed in contact with the other side of the membrane. The detecting liquid is also in contact with a ligand which has a higher affinity for the saccharides than for the polysaccharides. The concentration of saccharides is calculated from the measured change in the concentration of polysaccharides in the detecting liquid. A sensor for measuring the concentration of low molecular weight saccharides based on the same method is also described.

REFERENCES:
patent: 4073713 (1978-02-01), Newman
patent: 4344438 (1982-08-01), Schultz
patent: 4429122 (1984-01-01), Zupancic
patent: 4705503 (1987-11-01), Dorman et al.
Schultz et al, Biotechnology and Bioengineering Symp. 9, pp. 65-71 (1979).
Schultz et al, Diabetes Care, 5(3), pp. 245-253 (May-Jun. 1982).
Mansouri et al, Bio/Technology, pp. 885-890 (Oct. 1984).
Srinivasan et al, Biotechnology and Bioengineering, 28, pp. 233-239 (1986).
Schultz, Biomedical Applications. Synthetic Membranes: Science, Engineering and Applications, pp. 647-665 (1986).
The Merck Index, Merck & Co., Rahway, N.J., (1983), pp. 426, 780.

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