Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-09-03
1995-04-04
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435188, 435810, 435 794, 436518, 436 71, 436524, 436528, 436826, G01N 33544, G01N 33551, G01N 33577, G01N 33535
Patent
active
054037166
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates to a method for measuring the tissue factor in a specimen in high sensitivity and a kit used for measurement thereof. More specifically, this invention relates to a method for immunologically measuring the human tissue factor in a human specimen in high sensitivity by a sandwich method, and a kit used for measurement thereof.
2. Description of Related Art
A tissue factor is a lipoprotein which is involved in the blood coagulation reaction and has hitherto been called "tissue thromboplastin" or "coagulation factor III", and it is a kind of conjugated protein comprising a lipid part and the protein part (apoprotein).
Because of its extremely low content in living bodies and difficulty in purification, research into of the tissue factor on a molecular level has been delayed, but the cDNA of the apoprotein of the human tissue factor was cloned and the base sequence and amino acid sequence thereof were determined, in succession, in 1987 by three research groups (Spicel, E. R., et al. : Proc. Natl. Acad. Sci. USA 84, 5148 (1987), Morrissey, J. H., et al. : Cell 84, 129 (1987), Scarpati, E. M., et al., : Biochemistry 26, 5234 (1987). They report that the apoprotein of the human tissue factor comprises 263 amino acid residues and its molecular weight by the SDS-electrophoresis method is 47 KD.
Tissue factors exist in almost all the biotissues of various animals including human beings, and particularly, are distributed, in comparatively large quantities, in the circulatory system, for example in the brain, lungs and placenta, throughout which blood vessels are provided, and vascular endothelia, and usually, are expressed and exist as a membrane protein on the membrane of the cells of these tissues.
Further, their physiological action is to bear the initiation reaction of a series of extrinsic coagulation reactions caused by damages and breakdown of blood vessels. Specifically, in the presence of Ca.sup.++, they form a molecule complex with coagulation factor VII to activate both of coagulation factors X and IX, and as a result form fibrin gel in cooperation with intrinsic coagulation reaction. Namely, tissue factors as well as factor VII are proteins playing a central role in the initiation mechanism of extrinsic coagulation reaction. (Bach, R., et al. : Biochemistry 22, 4007 (1986), Fair, D. S., et al. : J. Boil. Chem. 262, 11692 (1987), Weiss, H. I., et al.,: Blood 73, 968 (1989)).
Tissue factors having the physiological actions described above have drawn attention as an important factor for blood coagulation. Thus, they play an extremely important role in researche in the basic medicine and clinical medicine of the circulatory system to measure easily and accurately a very small quantity of the human tissue factor in a specimen.
As methods to measure the human tissue factor in a specimen, several methods have hitherto been proposed. A representative example thereof is a method, called the method of Nemerson, to measure tissue factor activity by two stage steps (Nemerson, Y., J. Clin. Invest. 47 72 (1968). To put it briefly, the measurement method is one to judge tissue factor activity by adding to a specimen a mixed solution of coagulation factors VII and X to carry out reaction for a certain time, and then adding this reaction solution to normal plasma to which a crude phospholipid is added and measuring coagulation time. However, this method has a problem that since the stability of the reagents used is low and it is difficult to measure coagulation time with good accuracy, it is difficult to measure easily a very small quantity of the human tissue factor in a solution. Further, the above method is an indirect measurement method wherein tissue factor activity is judged by measuring coagulation factor Xa, and therefore, as a matter of course, there is a limit to its accuracy. Other tissue factor measurement methods proposed based on activity measurement have each the above problems.
On the other hand, the following are reported as imm
REFERENCES:
patent: 4148869 (1979-04-01), Deaton
patent: 4810630 (1989-03-01), Craig et al.
patent: 5017559 (1991-05-01), Dosako et al.
Derwent Abstract for JP48-37184.
P1530 Abstract for Kubota et al, Thromb. Haemostas. 54, 258, 1985.
Nemberson, Y., J. Clin. Invest. 47, 72-80, 1968.
Bjorklid, E., et al., Br. J. Haematol. 39, 445-459, 1978.
Andoh, K. et al., Thromb. Res. 43, 275-287, 1986.
Derwent Abstract for JP59-60260.
Derwent Abstract for JP-19766.
Spicer, E. K. et al., Proc. Natl. Acad. Sci. USA 84, 5148-5152, 1987.
Morrissey, J. H. et al., Cell 50, 129-135, 1987.
Scarpati, E. M. et al., Biochemistry 26, 5234-5238, 1987.
Bach , R. et al., Biochemistry 25, 4007-4020, 1986.
Fair, D. S. et al., J. Bio. Chem. 262, 11692-11698, 1987.
Weiss, H. J. et al., Blood 73, 968-975, 1989.
Calbiochem Corporation A Guide to the Properties and Uses of Detergents in Biology and Biochemistry (1988) pp. 4-12.
Hasegawa Ryoichi
Matsuzawa Kimihiko
Kepplinger Esther M.
Parsons Nancy J.
Teijin Limited
LandOfFree
Method for measurement of tissue factor in high sensitivity and does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for measurement of tissue factor in high sensitivity and , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for measurement of tissue factor in high sensitivity and will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2380010