Radiant energy – Luminophor irradiation – Methods
Patent
1992-11-19
1994-06-07
Hannaher, Constantine
Radiant energy
Luminophor irradiation
Methods
2504612, G01N 2164, G01N 2178
Patent
active
053192095
ABSTRACT:
A fluorescence probe dye is introduced into a cell, and excitation beams at three defferent wavelengths are irradiated to the cell to measure intensities of the fluorescence generated by the excitation beams, corresponding to the three wavelengths. Then, an equilibrium reaction equation for concentrations of the fluorescence probe dye, protein, free ions and their complexes in the cell, and a relationship equation between the fluorescence probe dye, protein, free ions and their complexes are used as simultaneous equations to give concentrations of the respective components. This process can correct interactions among various components of the cell due to bonding among them and a correct ion concentration can be determined.
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patent: 4900934 (1990-02-01), Peeters et al.
patent: 5149972 (1992-09-01), Fay et al.
Grynkiewicz et al., "A New Generation of Ca.sup.2+ Indicators with Greatly Improved Fluorescence Properties", Jour. Biological Chem., vol. 260, No. 6, Mar. 1985, pp. 3440-3450.
Hirano Masahiko
Kamiya Kiyoshi
Miyakawa Atsuo
Glick Edward J.
Hammamatsu Photonics K.K.
Hannaher Constantine
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