Method for measurement of cholesterol in high-density...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol

Reexamination Certificate

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Reexamination Certificate

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07811780

ABSTRACT:
The present invention provides a method, a reagent and a kit for the simple and accurate measurement of HDL cholesterol. The method for the measurement of cholesterol in high-density lipoprotein in a sample comprises reacting the sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising a specific nitrogen-containing surfactant having the structure of amine or ammonium salt and a polyanion, and measuring the formed hydrogen peroxide or reduced coenzyme.

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http://www.lookchem.com/cas-112/112-00-5.html, Jun. 30, 2010.

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