Method for massive directed mutagenesis

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification

Reexamination Certificate

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C435S006120, C536S023100, C536S024300

Reexamination Certificate

active

10362576

ABSTRACT:
The invention concerns the field of molecular biology and more particularly that of mutagenesis. It concerns a method of high-rate directed mutagenesis, that is the formation of numerous directed mutants in reduced time and with reduced number of steps. Said method is therefore referred to as massive mutagenesis.

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patent: 5830696 (1998-11-01), Short
patent: 6368861 (2002-04-01), Crameri et al.
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Stemmer, W.P. Rapid Evolution of a protein in vitro by DNA shuffling. Nature 370 : 389-391 (Aug. 1994).
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Picard et al. A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase. Nucleic Acids Resaearch 22(13) : 2587-2591 (1994).
New England Biolabs Catalog, p. 70 (1995).
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