Method for mass production of seedling of seed potato

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se

Reexamination Certificate

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C435S420000, C435S431000

Reexamination Certificate

active

07901938

ABSTRACT:
A method of mass producing potato seedlings, involves collecting growing points of seed potatoes and culturing the growing points in a liquid or solid medium; introducing in vitro plantlets obtained from the culture of the growing points to solid culture; and removing the in vitro plantlets from the solid culture, and planting through stem cutting and acclimatizing the in vitro plantlets in deep flow culture, in which a nutrient solution is circulating. Upon planting in hydroponic facilities, such potato seedlings have high adaptability to the external environment and thus rapidly, uniformly generate roots in a short time. The rapid root anchoring prevents planted seedlings from withering, leading to death, growing poorly, and the like. The direct planting of in vitro plantlets through stem cutting without a separate acclimatization process shortens the overall production period of potato seedlings by omitting the acclimatization process.

REFERENCES:
patent: 5498541 (1996-03-01), Oka et al.
patent: 5854066 (1998-12-01), Oka
patent: 5992090 (1999-11-01), Stutte et al.
Lee et al. “Effects of Quantum Flux Density on Photosynthesis and Chloroplast Ultrastructure in Tissue-Cultured Plantlets and Seedlings ofLiquidambar styracifluaL. towards Improved Acclimatization and Field Survival,” Plant Physiol. (1985) 78, pp. 637-641.
Akita et al., “Stimulation of potato (Solanum tuberosumL.) tuberization by semi continuous liquid medium surface level control,” Plant Cell reports (1994) 13:184-187.
Jiménez et al., “Improved production of potato microtubers using a temporary immersion system,” Plant Cell. Tissue and Organ Culture 69: 19-23, 1999.

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