Method for manufacturing xylitol with high-yield and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S183000, C435S189000, C435S252300, C435S254110, C435S254220, C536S023200

Reexamination Certificate

active

07745177

ABSTRACT:
The present invention provides a method for manufacturing xylitol with high-yield and high-productivity by using a xylitol dehydrogenase-deficient mutant of xylitol producing microorganism. This goal is achieved through modification of the metabolic pathway of the xylitol producing microorganism, preferably a natural xylose-assimilating yeasts and fungi, by disrupting or inactivating the expression of desired genes.

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Ko et al. Biotechnol Lett. Aug. 2006;28(15):1159-62. Epub Jun. 30, 2006.
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Kim et al., “Improvement of Xylitol Production by Controlling Oxygen Supply in Candida parapsilosis” J. of Fermentation & Bioengin. 83(3):267-270, 1997.
Ko et al., “Production of Xylitol from D-Xylose by a Xylitol Dehydrogenase Gene-Disrupted . . . ” Appl. & Environ. Microbiol. 72(6):4207-4213, 2006.
Ko et al., “Enhancement of xylitol productivity and yield using a xylitol dehydrogenase gene-disrupted mutant . . . ” Biotechnol. Lett. 28:1159-1162, 2006.
Kotter et al., “Isolation and Characterization of the Pichis stipitis xylitol dehydrogenase gene . . . ” Curr. Genet. 18:493-500, 1990.
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