Method for manufacturing minimal volume capsules containing biol

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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264 41, 264 43, 424424, 424493, 435382, 425 5, 514866, A61K 950, B01J 1320, C12N 506

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active

058277072

ABSTRACT:
The present invention provides methods and a device for producing minimal volume capsules containing viable cells or cellular aggregates. The methods and device use a two-phase aqueous emulsion system to form a dispersion of liquid capsule-forming materials in a continuous liquid phase to which is added a suspension of biological material. Alternatively, the biological material can be added to one or the other of the liquid phases. The composition of this emulsion is adjusted to promote the thermodynamically-driven process of particle engulfment by the dispersed droplets of liquid capsule-forming materials. Subsequently, the droplets engulf the biological material to form a liquid film surrounding the tissue and are converted to solid form, resulting in encapsulation of the biological material in minimum volume capsules.

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patent: 4927761 (1990-05-01), Reading et al.
patent: 5175093 (1992-12-01), Seifert
patent: 5529914 (1996-06-01), Hubbell et al.

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