Method for mammalian cell transfection using 25-hydroxycholester

Details Method for mammalian cell transfection using 25-hydroxycholester Method for mammalian cell transfection using 25-hydroxycholester

1992-06-17

1994-07-19

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 6, 435 721, 435948, 935 52, 935 55, C12N 1500, C12N 100, C12N 114, C12Q 168

Patent

active

053309049

ABSTRACT:
The present invention relates to a process, a cell culture medium, and a kit for the rapid, high-efficiency transfection of mammalian cells with exogenous DNA. The process comprises incubating a cell culture in the presence of a transfection medium comprising a serum that is different from the serum in the normal growth medium used to grow the cells, and DNA to produce transfected cells. In a preferred embodiment the normal growth medium comprises fetal bovine serum and the transfection medium comprises a serum such as human, calf, horse, lamb, or pig serum. The transfection medium may further comprise an hydroxylated sterol such as 25-hydroxycholesterol.

REFERENCES:
patent: 3884638 (1975-05-01), Dixon et al.
patent: 3954409 (1976-05-01), Hsia
patent: 4935363 (1990-06-01), Brown et al.
Sigmac Catalogue 1990, pp. 1889-1891, Sigma Chemical Company, Missouri.
Sigma Chemical Co. Catalogue 1990, pp. 305-306, 583, 1303-1323.
Documents Geigy-Scientific Tables, (1970), Diem, K. and Lentner, C. eds. pp. 600-604.
Chen et al., "High-Efficiency Transformation of Mammalian Cells by Plasmid DNA," Molec. and Cell. Biol., 2745-2752 (1987).

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