Method for making β-secretase co-crystals complexed...

Chemistry: analytical and immunological testing – Process or composition for determination of physical state... – Of crystal or crystalline material

Reexamination Certificate

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C435S183000, C117S927000

Reexamination Certificate

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07569391

ABSTRACT:
The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of β-secretase (BACE) polypeptide. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding human BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.

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