Chemistry: analytical and immunological testing – Process or composition for determination of physical state... – Of crystal or crystalline material
Reexamination Certificate
2006-12-06
2009-08-04
Weber, Jon P (Department: 1656)
Chemistry: analytical and immunological testing
Process or composition for determination of physical state...
Of crystal or crystalline material
C435S183000, C117S927000
Reexamination Certificate
active
07569391
ABSTRACT:
The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of β-secretase (BACE) polypeptide. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding human BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.
REFERENCES:
patent: 7217556 (2007-05-01), Benson et al.
patent: 2001/0016324 (2001-08-01), Gurney et al.
patent: 2001/0044521 (2001-11-01), Lin
patent: 2002/0055459 (2002-05-01), Chopra et al.
patent: 2004/0014194 (2004-01-01), Beyer et al.
patent: WO 01/00663 (2001-01-01), None
patent: WO 01/00665 (2001-01-01), None
patent: WO 02/25276 (2002-03-01), None
patent: WO 03/012089 (2003-02-01), None
Giege et al. (1994) Acta Cryst., D50, 339-350.
Branden et al (1999) Introduction to Protein Structure, Second Edition, Garland Publishing Inc., New York, pp. 374-375 and 382.
Drenth (1995) Principles of X-ray Crystallography, Springer, New York, p. 1.
Kierzek et al. (2001) Biophys Chem, 91:1-20.
Wiencek (1999) Ann Rev Biomed Eng., 1:505-534.
Hong et al., Structure of the protease domain of memapsin 2 (beta-secretase) complexed with inhibitor. Science, vol. 290, pp. 150-153 (2000).
Mallender et al., Characterization of recombinant, soluble beta-secretase from an insect cell expression system. Mol. Pharm., vol. 59, No. 3, pp. 619-626 (2001).
Bruinzeel et al., Recombinant insect cell expression and purification of human beta-secretase (BACE-1) for X-ray crystallography. Prot. Exp. and Pur., vol. 26, pp. 139-148 (2002).
Witkowski et al., Conversion of a beta-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamine. Biochemistry. Sep. 7, 1999;38(36):11643-50.
Lilie et al., Advances in refolding of proteins produced inE. coli. Curr Opin Biotechnol. Oct. 1998;9(5):497-501.
Kennedy et al., Measuring human beta-secretase (BACE1) activity using homogeneous time-resolved fluorescence. Anal Biochem. Aug. 1, 2003;319(1):49-55.
Wang et al., Crystallization of glycosylated human BACE protease domain expressed in Trichoplusia ni. Biochim Biophys Acta. May 6, 2004;1698(2):255-9.
Vassar et al., Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science. Oct. 22, 1999;286(5440):735-41.
Sinha et al., Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature. Dec. 2, 1999;402(6761):537-40.
Hussain et al., Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci. Dec. 1999;14(6):419-27.
Lin et al., Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc Natl Acad Sci U S A. Feb. 15, 2000;97(4):1456-60.
Creemers et al., Processing of beta-secretase by furin and other members of the proprotein convertase family. J Biol Chem. Feb. 9, 2001;276(6):4211-7.
Bennett et al., A furin-like convertase mediates propeptide cleavage of BACE, the Alzheimer's beta -secretase. J Biol Chem. Dec. 1, 2000;275(48):37712-7.
Esch et al., Cleavage of amyloid beta peptide during constitutive processing of its precursor. Science. Jun. 1, 1990;248(4959):1122-4.
Beyer Brian M.
Malcolm Bruce A.
Strickland Corey O.
Wang Wenyan
Wilson Eileen
Kim Alexander D
Schering Corporation
Weber Jon P
LandOfFree
Method for making β-secretase co-crystals complexed... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for making β-secretase co-crystals complexed..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for making β-secretase co-crystals complexed... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4132587