Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1979-07-09
1980-11-04
Warden, Robert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 68, 435113, 435265, 435272, C12P 2106, C12P 1312, C14C 100, C07G 700
Patent
active
042321239
ABSTRACT:
A method is disclosed for making a water-soluble hydrolyzate of a keratinaceous starting material which comprises first subjecting said starting material to acid treatment at a pH of 2 or below and at an elevated temperature above 80.degree. C. to effect mild hydrolysis thereof and then enzymatically degrading said acid-treated material in an aqueous bath in the presence of urea with an alkaline proteinase having an activity optimum in a range between pH 9 and pH 13, the initial pH of the enzymatic treatment being within the pH range optimum for the enzyme employed.
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D. A. Hall, "The Reaction Between Elastise and Elastic Tissue", Journal of Biochemistry, vol. 59, pp. 459-464, 1955.
Hoppe-Seyler/Thierfelder, Hardback der Physiologisch und Pathologisch-Chem Analyse, Tenth Ed., vol. II, p. 495, 1960.
A. L. Everett et al., Assay of Proteolytic Depilatory Enzymes on Keratin Activation with Metabisalfite, paper presented at Annual Meeting of Society of Amer. Bacteriologists, 1960.
Berg Alexander
Braeumer Klaus
Eckmayer Zdenek
Monsheimer Rolf
Pfleiderer Ernst
Firma Carl Freudenberg
R/hm GmbH
Warden Robert J.
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