Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2007-05-18
2008-08-12
Swope, Sheridan (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
C435S212000
Reexamination Certificate
active
07410775
ABSTRACT:
The present invention relates to the expression and secretion inSaccharomyces cerevisiaeof readily purifiable soluble variants of the Kex1 endopeptidase ofKluyveromyces lactisand the purification and use thereof for the in vitro processing of recombinant proteins usable in industrial applications. The soluble Kex1 endoproteases described here are free from the transmembrane domain of the native enzyme; the deletion of the transmembrane domain is achieved by removing at least 57 amino acid residues from the C-terminal.
REFERENCES:
patent: 0327377 (1989-08-01), None
Swiss-Prot Database Accession No. P09231, KEX1 protease precursor fromK. lactis, Mar. 1, 1989.
D. Germain, et al., Expression of theSaccharomyces cerevisiaeKex2p endoprotease in insect cells, Eur. J. Biochem. 204, 121-126 (1992).
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UniProt Database Accession No. P09231.
PIR—79 Database Accession No. KXBY from Mizuno et al Yeast KEX2 genes encodes an endopeptidase homologous to subtilisin-like serine proteases, Biohem Biophys Res Commun., Oct. 1988, 156(1), pp. 246-254, Alignment with Seq ID No. 2.
Issued Patents AA Database from US5,935,815 van den Ven et al. Mar. 8, 1999 Seq. ID No. 4, Alignment with Seq ID No. 2.
Orsini Gaetano
Taylor Geoffrey
Tonon Giancarlo
Tortora Paolo
Vanoni Marco
Bio-Ker S.R.L.
Darby & Darby P.C.
Swope Sheridan
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