Method for making a preconjugate

Chemistry: analytical and immunological testing – Biospecific ligand binding assay

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436543, 436547, 436816, 436817, 530345, 530403, 530404, 530405, 530406, 530408, 530409, 530410, G01N 3353

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active

058495991

ABSTRACT:
A method for making a preconjugate which includes immunogenic species of a polymorphic analyte. The method is carried out by reacting an activated binding moiety, and a polymorphic analyte at room temperature for between about 10 hours and about 60 hours. The attaching reaction results in an excess of the preconjugate which includes the immunogenic species of the polymorphic analyte. The preconjugate can be used to make an immunoreactive conjugate useful as a developer antigen in a competitive inhibition immunoassay for the polymorphic analyte.

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