Method for lysing Mycobacteria using achromopeptidase

Details Method for lysing Mycobacteria using achromopeptidase Method for lysing Mycobacteria using achromopeptidase

1991-06-24

1993-02-09

Yarbrough, Amelia B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435220, 4352531, 435270, 435 91, 536 231, C12Q 168, C12N 952, C12P 1934, C07H 2100

Patent

active

051852420

ABSTRACT:
The invention provides a rapid method for lysing Mycobacteria. In one embodiment is provided a method for lysing Mycobacteria which comprises exposing the bacteria to a lysis effective amount of the enzyme achromopeptidase.
The method of the invention is particularly advantageous since only one step is involved, it is expedient compared to prior methods, and little instrumentation is necessary. By practicing the present invention it is possible to lyse Mycobacteria with minimal effort. In addition, practicing the invention results in liberating cellular components including deoxyribonucleic acid (DNA) from Mycobacteria. Not only is DNA liberated, but the DNA is suited for subsequent analysis by way of probe hybridization, restriction enzyme analysis, and the like.

REFERENCES:
patent: 4900677 (1990-02-01), Hewitt
Zinsser Microbiology, 1984, Joklik et al., eds. Appleton-Century-Crofts, Norwalk, Conn., p. 12.
D. L. Whipple et al., J. Clin. Microbiol. 25:1511 (1987).
R. N. Picken et al., Molecular and Cellular Probes 2:289 (1988).
S. S. Hurley et al., J. Clin. Microbiol. 25:2227 (1987).
T. Ezaki et al., J. Clin. Microbiol. 16:844 (1982).

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