Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1990-03-05
1992-11-17
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436533, 436 71, 436800, 436829, G01N 33543, G01N 33546, G01N 3392
Patent
active
051643200
ABSTRACT:
A method for rapidly lysing liposomes having transition temperatures in the range of 35.degree. C. to 65.degree. C. is provided. Such liposomes are treated with a surfactant including polyethyleneglycol mono-n-alkyl ethers such as C.sub.12 H.sub.25 O(CH.sub.2 CH.sub.2 O).sub.n H where n=9-10, a nine mole ethylene oxide adduct of a blend of n-dodecanol, n-tetradecanol and n-hexadecanol, or other appropriate polyethyleneglycol mono-n-alkyl ether capable of causing rapid lysis. The method is applicable to fluorescence immunoassay procedures.
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Butt Abdul M.
Pollack Lewis
Readio Josephine D.
Rutner Herman
Sylvestre Marie M.
Becton Dickinson and Company
Nucker Christine M.
Preston David R.
Ronning, Jr. Royal N.
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