Method for lysing cells

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Lysis of micro-organism

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4353061, C12N 106

Patent

active

058375295

ABSTRACT:
This invention relates to a method for lysing cells. The method comprises simultaneously flowing a cell suspension and a lysis solution through a static mixer, wherein the cells exit the static mixer lysed. In another aspect of the present invention, the invention relates to a method for precipitating cell components, protein, and nucleic acids from a cell lysate or other solution containing precipitable material. The method comprises simultaneously flowing a cell lysate or other protein containing solution and a precipitating solution through a static mixer, wherein the lysate or protein solution exits the static mixer with its precipitable components precipitated. In another aspect of the present invention, the invention relates to a method where the two above-mentioned methods above are combined by using static mixers in series.

REFERENCES:
patent: 4296204 (1981-10-01), Grabner
patent: 4415670 (1983-11-01), Grabner

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