Method for low-temperature preservation of spermatozoa

Details Method for low-temperature preservation of spermatozoa Method for low-temperature preservation of spermatozoa

1988-06-21

1990-10-23

Rosen, Sam

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

A01N 102

Patent

active

049651862

ABSTRACT:
The method resides in cooling the spermatozoa in a high thermal-diffusive powdered material that has preliminarily been cooled down to the refrigerant temperature. Cooling is carried out in a medium of a cryopreservative, containing a salt buffer, an antishock component, and a cryoprotector. The cryoprotector is taken in a concentration high enough for vitrification to develop in the spermatozoa. An example of the high thermal diffusive powdered material is alumina.

REFERENCES:
patent: 3185623 (1965-05-01), Smith et al.
patent: 3791384 (1974-02-01), Richter et al.
Sherman-Fertility and Sterility vol. 14, No. 1 (1963), pp. 49-64.
Nauk et al.-Chem. Abst. vol. 102 (1985), p. 110, 595e.

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