Method for low-temperature preservation of embryos

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor

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A01N 102

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049651854

ABSTRACT:
The method consists in cooling of embryos down to the temperature of the refrigerant in a buffer-salt fluid, the cooling process being carried out in a temperature diffusive powdered material that has preliminarily been cooled down to the refrigerant temperature. The buffer-salt fluid comprises a mixture of cryoprotectors, i.e., glycerol, dimethylsulphoxide, and a dihydric alcohol. An example of the temperature diffusive material is powdered metal.

REFERENCES:
patent: 4474016 (1984-10-01), Winchell
patent: 4494385 (1985-01-01), Kuraoka et al.
patent: 4688387 (1987-08-01), Conaway
Theriogenology, 1985, vol. 23, pp. 235 & 199.
Experimental Cell Research, vol. 89, No. 1, Nov. 1974, pp. 79-88.
Theriogenology, 1986, vol. 25, pp. 6-10.
Cryobiology-vol. 16, No. 1 (Feb. 1979), p. 6.

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