Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1985-08-29
1988-12-27
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 7, 435803, 436 63, 436501, 514 44, 536 27, 935 6, 935 9, 935 19, 935 23, 935 78, 935 81, C12Q 168, G01N 3348
Patent
active
047940751
ABSTRACT:
A method for distinguishing fragments of DNA which contain single base mismatches from their perfectly paired homologues is disclosed. Single stranded regions within a duplex fragment are modified with carbodiimide, which reacts with unpaired guanine (G) and thymine (T) residues in DNA. Linear duplex DNA molecules do not react, while DNA molecules with single base mismatches react quantitatively with carbodiimide. Following reaction with carbodiimide, the DNA molecules are fractionated on high percentage polyacrylamide gels such that modified and unmodified fragments can be clearly distinguished. Application of this technique in order to located and purify DNA sequence differences responsible for phenotype variation and inherited disease is disclosed.
REFERENCES:
Hale, P. et al., "Carbodiimide Inactivation of Escherichia Coli . . . ", J. Biol. Chem. 258 (12), 7828-7839 (Jun. 25, 1983).
Proc. Nat'l Acad. Sci. USA 72: 989-993 (1975) T. E. Shenk, et al., "Biochemical Method for Mapping Mutational Alterations in DNA with S1 Nuclease: The Location of Deletions and Temperature-Sensitive Mutations in Simian Virus 40".
Biochemistry 15: 4457-63 (1976) D. Kowalski, et al., "Mung Bean Nuclease I. Physical, Chemical, and Catalytic Properties".
Proc. Nat'l Acad. Sci. USA 80: 1579-83 (1983) S. G. Fischer and L. S. Lerman, "DNA Fragments Differing by Single Base-Pair Substitutions Are Separated in Denaturing Gradient Gels: Correspondence with Melting Theory".
Nucl. Acid. Res. 9: 3647-56 (1981) R. B. Wallace, et al., "Oligo Nucleotide Directed Mutagenesis of the Human .beta.-Globin Gene: A General Method for Producing Specific Point Mutations in Cloned DNA".
J. Virol. 18: 205-210 (1976) J. Lebowitz, et al., "Chemical Modification of Simian Virus 40 DNA by Reaction with a Water-Soluble Carbodiimide".
Nucl. Acid. Res. 4: 1695-1711 (1977) J. Lebowitz et al., "Carbodiimide Modification of Superhelical PM2 DNA: Considerations Regarding Reaction at Unpaired Bases and the Unwinding of Superhelical DNA with Chemical Probes".
Nucl. Acid Res. 8: 4521-34 (1980) J. M. Kelly and B. E. H. Maden, "Chemical Modification Studies and The Secondary Structure of HeLa Cell 5.8S rRNA".
Biochemistry 6: 3632-39 (1967) N. W. Y. Ho and P. T. Gilham, "The Reversible Chemical Modification of Uracil, Thymine, and Guanine Nucleotides and the Modification of the Action of Ribonuclease on Ribonucleic Acid".
Proc. Nat'l Acad. Sci. USA 78(11): 6613-17 (1981) S. V. Suggs, et al. "Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human .beta..sub.2 -Microglobulin".
Proc. Nat'l Acad. Sci. USA 80(1): 278-82 (1983) B. J. Conner, et al., "Detection of Sickle Cell .beta..sup.5 -Globin Allele by Hybridization with Synthetic Oligonucleotides".
Proc. Nat'l. Acad. Sci. USA 80(7): 2007-11 (1983) D. H. Schulze, et al., "Comparison of the Cloned H-2K.sup.bml Variant Gene with the H-2K.sup.b Gene Shows a Cluster of Seven Nucleotide Differences".
Biochemistry 16(24): 5329-41 (1977) D. E. Kohne, et al., "Room Temperature Method for Increasing the Rate of DNA Reassociation by Many Thousandfold: The Phenol Emulsion Reassociation Technique".
Biopolymers 21: 665-677 (1982) R. Wieder and J. G. Wetmur, "Factors Affecting the Kinetics of DNA Reassociation in Phenol-Water Emulsion at High DNA Concentrations".
J. Org. Chem. 21: 439-441 (1956) J. C. Sheehan et al., "The Use of Water-Soluble and Basic Carbodiimides in Peptide Synthesis".
J. Org. Chem. 26: 2525-28 (1961) J. C. Sheehan, et al., "A Convenient Synthesis of Water-Soluble Carbodiimides".
Gene 26: 171-179 (1983) W. A. M. Loenen and F. R. Blattner, "Lambda Charon Vectors (CH 32, 33, 34 and 35) Adapted for DNA Cloning in Recombination-Deficient Hosts".
Nature 302: 33-37 (1983) D. J. Capon, et al., "Complete Nucleotide Sequences of the T24 Human Bladder Carcinoma Oncogene and its Normal Homoloque".
Proc. Nat'l Acad. Sci. USA 81: 4008-12 (1984) O. Fasano, et al., "Analysis of the Transforming Potential of the Human H-ras Gene by Random Mutagenesis".
Proc. Nat'l Acad. Sci. USA 74: 5463-67 (1977) F. Sanger, et al., "DNA Sequencing with Chain-Terminating Inhibitors".
Anal. Biochem. 114: 193-197 (1981) D. S. Holmes and M. Quigley, "A Rapid Boiling Method for the Preparation of Bacterial Plasmids".
J. Mol. Biol. 19: 548-555 (1966) F. H. C. Crick, "Codon-Anticodon Pairing: The Wobble Hypothesis".
J. Biol. Struct. and Dynamics 1: 509-521 (1983) P. Lu, et al., "Possible Molecular Detent in the DNA Structure at Regulatory Sequences".
Nature 304: 230-34 (1983) V. J. Kidd, et al., ".alpha..sub.1 -Antitrypsin Deficiency Detection by Direct Analysis of the Mutation in the Gene".
Casna Nancy J.
Ford John P.
Novack David F.
Lifecodes Corporation
Wagner Richard
Warden Robert J.
LandOfFree
Method for locating and purifying DNA containing single base mis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for locating and purifying DNA containing single base mis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for locating and purifying DNA containing single base mis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-867916