Method for ligating nucleic acids and molecular cloning

Details Method for ligating nucleic acids and molecular cloning Method for ligating nucleic acids and molecular cloning

2006-09-19

2006-09-19

Ketter, James (Department: 1636)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C435S462000, C435S455000, C435S091410

Reexamination Certificate

active

07109178

ABSTRACT:
The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.

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