Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1983-06-22
1985-10-29
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 4, 435188, 435810, G01N 3354, C12Q 100, C12N 996
Patent
active
045500754
ABSTRACT:
Methods and compositions are disclosed for the qualitative and quantitative identification of specific ligand in a fluid medium suspected of containing the ligand. The method of the present invention involves the formation of a liquid reaction mixture comprising an enzyme, a ligand-specific binding component, a conjugate comprising ligand coupled to a modulator label, and sample fluid medium suspected of containing ligand. Once the reaction mixture is formed, enzyme activity is monitored and the presence and amount of ligand in the sample fluid medium is determined by changes in enzyme activity.
Novel compositions useful in practicing the disclosed methods are provided. These include a biotin binding protein enzyme modulator, novel ligand-enzyme modulator conjugates, and biotin containing enzymes.
The methods and compositions of the present invention are particularly useful in the qualitative and quantitative identification of pharmaceuticals and any such antigenic units or sites, including haptens and fragments of large molecules present in such biologic fluids as blood, urine, spinal fluid, amniotic fluid, lymphatic fluid and the like.
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Bacquet Cathy A.
Twumasi Daniel Y.
Kallestad Laboratories, Inc.
Nucker Christine M.
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