Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1997-03-03
1999-07-13
Campell, Bruce R.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
435410, 435420, C12N 500
Patent
active
059225998
ABSTRACT:
Plantlets of trees of genus Gmelina can be efficiently regenerated on a large scale by culturing shoot apexes of trees of genus Gmelina on a B5 medium or its modified medium, each containing benzylaminopurine (BAP) or indole-3-butyric acid (IBA) together with BAP as plant growth regulators, thereby inducing and proliferating multiple shoots each having a large number of either definite buds or adventitious buds or both, then producing a large number of shoots from the resulting multiple shoots, transplanting the shoots to a B5 medium or its modified medium containing no plant growth regulator or containing both BAP and IBA as plant growth regulators; and the regenerated plantlets can be efficiently acclimatized to outdoor conditions by transplanting the regenerated plantlets to a mixture of river sands and crushed brick pieces in a mixing ratio of 1:1 by volume and acclimatizing the transplanted plantlets to outdoor conditions in an acclimatization box; and in this manner, trees of genus Gmelina can be propagated on a large scale.
REFERENCES:
Murashige, T. and Skoog, F., "A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures," Physiolgia Plantarum, vol. 15, 1962, pp. 473-497.
Gamborg, O.L., et al., "Nutrient Requirements of Suspension Cultures of Soybean Root Cells," Experimental Cell Research, vol. 50, 1968, pp. 151-158.
Yang et al., Micropropagation of Gmelina Aborea R., Proceedings of the SABRAO International Symposium 29, 1992, pp. 213-218.
Crizaldo, Tissue Culture Of Fast-Growing Trees, Sylvatrop Philipp. For. Res. J. 5(2): (1980) pp. 123-137.
SK Roy et al (1992) In Vitro Cellular & Developmental Biology 28(3, pt. II): 116A.
HT Hartmann et al (1975) Plant Propagation Principles and Practices pp. 17-52.
SH Mantell et al (1985) Principles of Plant Biotechnology pp. 130-157, 227, 230.
Nakamura Kentaro
Soda Ryo
Campell Bruce R.
Sumitomo Forestry Co., Ltd.
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