Method for labeling oligonucleotide with ammonia-sensitive ligan

Details Method for labeling oligonucleotide with ammonia-sensitive ligan Method for labeling oligonucleotide with ammonia-sensitive ligan

1997-03-05

1998-05-26

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 253, C07H 102, C07H 2100, C07H 2104

Patent

active

057567050

ABSTRACT:
The invention provides method for labeling oligonucleotides and their analogs with ammonia-sensitive ligands. A non-ammonia cocktail is used for cleavage and deprotection in combination with base labile linkers. The labeling process is done on the solid phase and cleaved by the non-ammonia cocktail to prevent the ligand from decomposition.

REFERENCES:
patent: 4965349 (1990-10-01), Woo et al.

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