Method for labeling DNA and RNA

Details Method for labeling DNA and RNA Method for labeling DNA and RNA

2007-04-24

2007-04-24

Kim, Young J. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

10057753

ABSTRACT:
A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.

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patent: 6818398 (2004-11-01), Bavykin et al.
patent: 2003/0096229 (2003-05-01), Bavykin et al.
patent: WO 9922020 (1999-05-01), None
Rosenthal et al., “Automated sequencing of fluorescently labelled DNA by chemical degradation,” DNA Sequence, 1990, vol. 1 No. 1, pp. 63-71.

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