Method for labeling antibodies with a metal ion

Details Method for labeling antibodies with a metal ion Method for labeling antibodies with a metal ion

1990-10-19

1993-01-05

Nucker, Christine M.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

5303913, 530402, 530404, 530408, 534 10, 534 14, 436545, 436547, 436548, 435810, 424 11, C07K 1528, C07K 1702, A61K 4902

Patent

active

051771926

ABSTRACT:
Methods for rapidly labeling sulfhydryl-containing antibodies or fragments with technetium or rhenium radioisotopes are disclosed. The method generally involves combining an aqueous mixture of the radiometal in an oxidized form, a reducing agent and a water-soluble polyhydroxycarbonylic acid ligand to form a stable complex of the radiometal in its reduced state and contacting the mixture with the sulfhydryl-containing antibody or fragment to produce a radiometal-labeled antibody or fragment.

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