Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2006-03-14
2006-03-14
Achutamurthy, Ponnathapu (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S106000, C435S183000, C435S232000, C435S252100, C435S252300, C435S252330, C435S320100, C536S023100, C536S023200
Reexamination Certificate
active
07011961
ABSTRACT:
A method of producing L-threonine using a microorganism is provided, one or more copies of each of the phosphoenolpyruvate carboxylase gene and the threonine operon are additionally intedgrated into a particular site of the chromosomal DNA of the microorganism, whiel its inherent phophoenolpyruvate carboxylase gene and threonine operon remain.
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“pBRINT-Ts: a plasmid family with a temperature-sensitive replicon, designed for chromosomal integration into the lacZ gene ofEscherichia coli”; Authors: Sylvie Le Borgne, Beatriz Palmeros, Fernando Valle, Francisco Bollvar and Guillermo Gosset; Elsevier; Gene An International Journal on Genes and Genomes, vol. 223; 1998; pp. 213-219.
“L-Threonine production by L-aspartate- and L-homoserine-resistant mutant ofEscherichia coli”; Authors: Satoru Furukawa, Akio Ozaki and Toshihide Nakanishi; Applied Microbiology and Biotechnology, vol. 29; 1988; pp. 550-553.
Kim Dai-Chul
Kim Yeong-Chul
Lee Jin-Ho
Noh Kap-Soo
Ok Seung-Han
Achutamurthy Ponnathapu
Cantor & Colburn LLP
Cheil Jedang Corporation
Fronda Christian L.
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