Method for knockout mutagenesis in Streptococcus pneumoniae

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

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435488, 4352523, 43525233, C12N 1500, C12N 1531, C12N 1587

Patent

active

059812818

ABSTRACT:
Provided herein is a method to produce knockout mutations at targeted sites in the genome of Streptococcus pneumoniae.

REFERENCES:
patent: 5747281 (1998-05-01), Shuler et al.
Kolkman et al., J. Bacteriology 178(13):3736-3741 (1996).
Claverys et al., Gene 164:123-128 (1995).
Tao et al., Gene 120:105-110 (1992).
K. F. Chater and C. J. Bruton. "Mutational cloning in Streptomyces and the isolation of antibiotic production genes." Gene 26(1):67-78 (1983).

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