Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-07-31
2000-01-18
Brusca, John S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435810, C12Q 168, C12P 1934
Patent
active
060156767
ABSTRACT:
The present invention provides a simple, specific and nontoxic gene knock-out method by formation of covalently bonding between modified probes and targeted sequences. When covalently modified first strand probes are hybridized with a second strand of targeted gene transcripts, certain modified bases of said first strand will interact with natural bases of said second strand to form covalent bonds by which the translation of said second strand is inhibited. Because the hybridization of said two strands generates covalent base-pairing only between their complementary homology region(s), such specificity increases the targeting efficiency of a gene knock-out system. Also, because neither a polymerase extension reaction nor a nuclease digestion can be performed through the covalently bonded region(s) of aforesaid hybrids, the present invention in conjunction with a delivery method can be used to inactivate intracellular functions of targeted nucleotide sequences, to inhibit viral infections in vivo and to increase binding stability of antisense drugs in a gene therapy.
REFERENCES:
patent: 4599303 (1986-07-01), Yabusaki et al.
patent: 4689320 (1987-08-01), Kaji
patent: 4806463 (1989-02-01), Goodchild et al.
patent: 5023243 (1991-06-01), Jullis
patent: 5030557 (1991-07-01), Hogan et al.
patent: 5110802 (1992-05-01), Cantin et al.
patent: 5589339 (1996-12-01), Hampson et al.
patent: 5591575 (1997-01-01), Hampson et al.
patent: 5739309 (1998-04-01), Dattagupta et al.
Falletta et. al., "Phase 1 Evaluation of Kdiaziquone in Childhood Cancer", Investigational new Drugs 8: 167-170 (1990).
Hartley et. al., DNA Cross-Linking and Sequence Selectivity of Aziridinylbenzoquinones, biochemistry 30: 11719-11724 (1991).
Hampson et.al., "Chemical Crosslinking Subtraction; A New Method for the Generation of subtractive Hybridiztion Probes", Nucleic Acids Res. 20: 2899 (1992).
Kimler et.al., "Combination of Aziridinylbenzoquiinone and Cis-platinum with Radiation Therapy in the 9L Rat Brain Tumor model", International Journal of Radiation Oncology, Biology, Physics 26:445-450 (1993).
Majumdar et.al., "Stepwise nMechanismof HIV Reverse Transcriptae: Primer Funtional of Phosphorothioate Oligodeoxynucleotide", Biochemistry 28: 1340-1346 (1989).
Matsukura et.al., "Regulation of viral expression of human immunodeficiency virus in vitro by an antisense phosphorothioate against rev (art/trs) in chronically infected cells", Proc. Natl. Acad. Sci. 86:4244-4248 (1989).
Sambrook et.al., "Molecular Clonging, 2nd Edition", Gold Spring Harbor Laboratory Press (1989).
Solomons et.al., "orgnaic Chemistry, 6th Edition", John Wiley & Sons Press (1996).
Tan et.al., "Phase 1 Study of Aziridinylbenzoquinone in Children with Cancer", Cancer Research 44:831-835 (1984).
Lin Shi-Lung
Ying Shao-Yao
Brusca John S.
Chan Raymond Y.
Epiclone Inc.
Wang Andrew
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