Method for kit for estimating the amount of methyl anthranilate

Details Method for kit for estimating the amount of methyl anthranilate Method for kit for estimating the amount of methyl anthranilate

1993-01-08

1993-10-05

Housel, James C.

Chemistry: analytical and immunological testing

Nitrogen containing

Amine and quaternary ammonium

436106, 436 19, 422 61, G01N 3300

Patent

active

052504417

ABSTRACT:
A method and kit for estimating the amount of methyl anthranilate includes obtaining a sample suspected of having methyl anthranilate, acidifying the sample with a strong acid, adding sodium nitrite to the acidified sample producing nitrous acid which in the presence of methyl anthranilate produces a diazonium ion. The method further comprises a step of adding sulfamic acid which removes excess nitrous acid from the sample, followed by the addition N-(1-naphthyl) ethylenediamine which reacts with the diazonium ion to form a colored complex which is color responsive which is then compared to standards prepared using known amounts of methyl anthranilate. The kit provides the a sample holder and vials of ingredients and standards to practice the method.

REFERENCES:
patent: 3907769 (1975-09-01), Dehnert et al.
patent: 5200330 (1993-04-01), Page et al.

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