Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Patent
1994-12-14
1998-09-01
Marx, Irene
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
435171, 435 711, 435814, 435911, 435917, 435942, 4352568, 4352541, 435244, 530412, 530823, 530824, 424605, C12N 900, C12P 2100
Patent
active
058010343
ABSTRACT:
This invention provides a method for killing cells in fermentation mixtures in order to prepare the fermentation mixture for processing to recover or extract a desired product from the fermentation mixture. A preferred method of this invention comprises in either order, adjusting the pH of the fermentation mixture to a value equal to or less than about two pH units below the pK.sub.a of the compatible organic acid using a mineral acid, and adding a sufficient amount of a compatible organic acid and/or organic acid salt to the mixture to effect a substantially complete cell kill. The method of this invention is useful for killing microorganisms such as yeast, bacteria or fungi in any culture or fermentation mixture and is particularly useful in systems where it is desired to kill the cells without lysing them.
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Baliu Enrique F.
Heinsohn Henry G.
Lawlis, Jr. Virgil B.
Anderson Kirsten A.
Genencor International
Marx Irene
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