Method for isolation of unclipped HIV envelope protein

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

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53038835, 530412, C07K 122

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active

056749848

ABSTRACT:
Methods for producing and isolating unclipped HIV env proteins are provided. According to the methods, an antibody directed to an HIV epitope spanning the env clip site is used to selectively separate unclipped HIV env protein from clipped env protein.

REFERENCES:
patent: 4725669 (1988-02-01), Essex et al.
patent: 5166050 (1992-11-01), Shriver et al.
Fox, J.L., "No Winners Againts AIDS," Bio/Technology 12:128, Feb. 1994.
Allan, et al., "Major glycoprotein antigens that induce antibodies in AIDS patients are encoded by HTLV-III" Science 228 (4703):1091-1094 (May 1985).
Barin et al., "Virus Envelope Protein of HTLV-III Represents Major Target Antigen for Antibodies in AIDS Patients" Science 228:1094-1096 (1985).
Barrett et al., "Large-Scale Production and Purification of a Vaccinia Recombinant-Derived HIV-1 gp160 and Analysis of Its Immunogenicity" AIDS Research and Human Retroviruses 5(2):159-171 (1989).
Celltech, "CHO cell rgp120" Cerificate of Analysis pp. 1-2 (01/ 222, 1990).
Clements et al., "The V3 Loops of the HIV-1 and HIV-2 Surface Glycoproteins Contain Proteolytic Cleavage Sites; A Possible Function in Viral Fusion" AIDS Research and Human Retroviruses 7:3-16 (1991).
Kitchen et al., "Aetiology of AIDS--antibodies to human T-cell leukaemia virus (type III) in haemophiliacs" Nature 312:367-369 (1984).
Krust et al., "Characterization of a monoclonal antibody specific for the HIV-1 precursor glycoprotein" AIDS 2:17-24 (1988).
Robey et al., "Prospect for prevention of human immunodeficiency virus infection: Purified 120-kDa envelope glycoprotein induces neutralizing antibody" Proc. Natl. Acad. Sci. 83:7023-7027 (1986).
Robey, W.G. et al., "Characterization of envelope and core structural gene products of HTLV-III with sera from AIDS patients" Science 228 (4699):593-595 (May 3, 1985).
Veronese et al., "Characterization of gp41 As the Transmembrane Protein Coded by the HTLV-III/LAV-Envelope Gene" Science 229:1402-1405 (1985).
Anderson et al., "Effect of Dose and Immunization Schedule on Immune Response of Baboons to Recombinant Glycoprotein 120 of HIV-1", Jour. of Infectious Diseases, 160(6):960-969 (1989).
Arthur et al., "Challenge of Chimpanzees (Pan troglodytes) Immunized with Human Immunodeficiency Virus Envelope Glycoprotein gp120", Jour of Virology, 63(12): 5046-5053 (1989).
Berman et al., "Expression and Immunogenicity of the Extracellular Domain of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein, gp160", Jour of Virology, 63(8): 3489-3498 (1989).
Berman et al., "Human immunodeficciency virus type 1 challenge of chimpanzees immunized with recombinant envelope glycoprotein gp120", Proc Natl Acad Sci USA, 85:5200-5204 (1988).
Berman et al., "Protection from Genical Herpes Simplex Virus Type 2 Infection by Vaccination with Cloned Type 1 Glycoprotein D: ", Science, 227:1490-1492 (1985).
Berman et al., "Protection of chimpanzees from infection by HIV-1 after vaccination with recombinant glycoprotein gp120 but not gp160", 345:622-625(1990).
Desrosiers et al., "Vaccine protection against simian immunodeficiency virus infection", Proc Natl Acad Sci USA, 86:6353-6357 (1989).
Fahey et al., "Status of immune-based therapies in HIV infection and AIDS", Clin. exp. Immunol., 88:1-5 (1992).
Fauci et al., "Development and Evaluation of a Vaccine for Human Immunodeficiency Virus (HIV) Infection", Annals of Internal Med, 110(5):373-385 (1989).
Homsy et al., "The Fc and Not CD4 Receptor Mediates Antibody Enhancement of HIV Infection in Human Cells", Science, 244:1357-1360 (1989).
Hu et al., "Effect of immunization with a vaccina-HIV env recombinant on HIV infection of chimpanzees", Nature, 328:721-723 (1987).
Hu et al., "Expression of AIDS virus envelope gene in recombinant vaccinia viruses", Nature, 320:537-540 (1986).
Jahaverian et al., "Principal neutralizing domain of the human immunodeficiency virus type 1 envelope protein", Proc Natl Acad Sci USA, 86:6768-6772 (1989).
Lasky, Laurence A., "Current Status of the Development of an AIDS Vaccine", Crit Revs in Immunology, 9(3):153-172 (1989).
Lasky, et al., "Delineation of a Region of the Human Immunodeficiency Virus Type 1 gp120 Glycoprotein Critical for Interaction with the CD4 Receptor", Cell, 50:975-985 (1987).
Lasky et al., "Neutralization of the AIDS Retrovirus by Antibodies to a Recombinant Envelope Glycoprotein", Science, 233:209-212 (1986).
Letvin et al., "AIDS-like Disease in Macaque Monkeys Induced by Simian Immunodeficiency Virus: A Vaccine Trial", Vaccines, 87:209-213 (1987).
Looney et al., "Type-Restricted Neutralization of Molecular Clones of Human Immunodeficiency Virus", Science, 241:357-359 (1988).
Matsushita et al., "Characterization of a Human Immunodeficiency Virus Neutralizing Monoclonal Antibody and Mapping of the Neutralizing Epitope", Jour of Virology, 62(6):2107-2114 (1988).
Modrow et al., "Computer-Assisted Analysis of Envelope Protein Sequences of Seven Human Immunodeficiency Virus Isolates: Prediction of Antigenic Epitopes in Conserved and Variable Regions", Jour of Virology, 61(2):570-578 (1987).
Murphy-Corb et al., "A Formalin-Inactivated Whole SIV Vaccine Confers Proection in Macaques", Science, 2:1293-1297 (1989).
Newmark, Peter, "Receding Hopes of AIDS vaccines", Nature, 333:699 (1988).
Palker et al., "Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic pepides", Proc Natl Acad Sci USA, 85:1932-1936 (1988).
Prince et al., "Failure of a human immunodeficiency virus (HIV) immune globulin to protect chimpanzees against experimental challenge with HIV", Proc Natl Acad Sci USA, 85:6944-6948 (1988).
Robinson, Jr. et al., "Antibody-Dependent Enhancement of Human Immunodeficiency Virus Type 1 Infection", The Lancet, 790-794 (1988).
Robinson, Jr. et al., "Human monoclonal antibodies to the human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 enhance HIV-1 infection in vitro", Proc Natl Acad Sci USA, 87:3185-3189 (1990).
Rusche et al., "Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120", Proc Natl Acad Sci USA, 85:3198-3202 (1988).
Salk et al., "Control of Influenza and Poliomyelitis with Killed Virus Vaccines", Science, 195:834-847 (1977).
Salk Jonas, "Prospects for the control of AIDS by immunizing seropositive individuals", Nature, 327:473-476 (1987).
Stephens et al., "A chink in HIV's armour?", Nature, 343:219 (1990).
Vandenbark et al., "Immunization with a synthetic T-cell receptor V-region peptide protects against experimental autoimmune encephalomyelitis", Nature, 341:541-544 (1989).
Van Eendenburg et al., "Cell-Mediated Immune Proliferative Responses to HIV-1 of Chimpanzees Vaccinated with Different Vaccinia Recombinant Viruses", Aids Res and Human Retroviruses, 5(1):41-50 (1989).
Zagury et al., "A group specific anamnestic immune reaction against HIV-1 induced by a candidate vaccine against AIDS", Nature, 332:728-731 (1988).
Zaguary et al., "Immunization against AIDS in humans", Nature, 326:249-250 (1987).
Zarling et al., "T-cell responses to human AIDS virus in macaques immunized with recombinant vaccinia viruses", Nature, 323:344-346 (1986).
Chakrabarti et al., "Expression of the HLTV-III envelope gene by a recombinant vaccinia virus", Nature, 320:535-540 (1986).

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