Method for isolation of silymarin from Sylibum marianum seeds

Drug – bio-affecting and body treating compositions – Plant material or plant extract of undetermined constitution... – Containing or obtained from a seed or nut

Reexamination Certificate

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C424S725000

Reexamination Certificate

active

07318940

ABSTRACT:
This invention relates to a method for the isolation of silymarin fromsilybum marianumseeds without precooling wherein the ground seeds are defatted using a hydrocarbon solvent, followed by extraction with a nontoxic medium polarity solvent, purification of the dried extract and drying of the crystals thus obtained.

REFERENCES:
patent: 3773932 (1973-11-01), Madaus
patent: 4368195 (1983-01-01), Madaus
patent: 4895839 (1990-01-01), Bombardelli
patent: 6262019 (2001-07-01), Keller et al.
patent: 6309678 (2001-10-01), Tandon
patent: 7172774 (2007-02-01), Leko
patent: 2017789 (1971-10-01), None
patent: 112261 (1975-04-01), None

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