Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Patent
1985-11-13
1988-08-23
Kight, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
530408, 530409, 530344, 530345, 530412, 530417, 530370, C07K 312
Patent
active
047662050
ABSTRACT:
Disclosed are improved methods for orienting polypeptide molecules containing multiple disulfide bonds into their native conformation through (1) treating the polypeptide with (a) a denaturing agent and (b) a reducing agent capable of reductively dissociating cystine disulfide bonds; (2) concurrently reducing the concentration of the reducing agent while forming a stable intermediate by reacting a disulfide containing compound with the reduced cysteine moieties of the polypeptide and; (3) forming native cystine disulfide bonds in the presence of a mild oxidizing/reducing environment while dissociating the disulfide-group polypeptide moeities.
REFERENCES:
patent: 4511502 (1985-04-01), Builder et al.
patent: 4511503 (1985-04-01), Olson et al.
patent: 4512922 (1985-04-01), Jones et al.
patent: 4518526 (1985-05-01), Olson
patent: 4530787 (1985-07-01), Shaked et al.
patent: 4569790 (1986-02-01), Koths et al.
Edens et al., "Cloning of CDNA Encoding . . . Thaumatin . . . E. Coli", Gene, 18, 1982, pp. 1-12.
Orsini et al., IBC 253(10), 1978, pp. 3453-3458.
Klausner, "And Then There Were Two", Biotechnology, vol. 3, pp. 605-614, Jul. (1985).
Lehninger, Principles of Biochemistry, 177-179 (1982).
Odorzynski and Light, "Refolding of Mixed Bovine Trypsinogen and Glutathione", J. Biol. Chem., vol. 254, No. 10, pp. 4291-4295 (1979).
Smithies, "Disulfide-Bond Cleavage and Formation in Proteins", Science, vol. 150, pp. 1595-1598 (1965).
Stryer, Biochemistry, 32-36 (2d Ed. 1981).
Wetzel et al., "Expression in Escherechia coli of a Chemically Synthesized Gene for a `Mini-C` Analog of Human Proinsulin", Gene, 16, pp. 63-71 (1981).
Beatrice Companies, Inc.
Draper Garnette D.
Kight John
LandOfFree
Method for isolation of recombinant polypeptides in biologically does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for isolation of recombinant polypeptides in biologically, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for isolation of recombinant polypeptides in biologically will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-836556