Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1996-05-24
1998-12-29
Patterson, Jr., Charles L.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530413, 530416, 530381, C07K 118, C07K 122, C07K 114
Patent
active
058544038
ABSTRACT:
The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt. The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant rvWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active. Further, the invention relates to a pharmaceutical preparation that contains rvWF, which comprises mulitimers with a high structural integrity.
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Den-Bouwmeester Renate
Dorner Friedrich
Eibl Johann
Falkner Falko-Guenter
Fischer Bernhard
Immuno Aktiengesellschaft
Patterson Jr. Charles L.
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