Method for isolation of connective tissue biomatrix

Details Method for isolation of connective tissue biomatrix Method for isolation of connective tissue biomatrix

1983-06-06

1987-02-10

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435267, 435273, C12N 500

Patent

active

046422927

ABSTRACT:
A method is disclosed for isolation of connective tissue fibers, called biomatrix, containing a significant portion of the extracellular matrix, i.e., basement membrane components and components of the ground substance. The connective tissue fibers isolated by this method provide significantly higher survival and attachment rates, and often significantly improved growth properties, for in vitro cultures of differentiated cells, especially epithelial cells, over current culture substrates which do not contain these fibers.

REFERENCES:
patent: 3660566 (1972-05-01), Vinson et al.
patent: 4264155 (1981-04-01), Miyata
patent: 4288557 (1981-09-01), Karkhavis et al.
Meegan et al., Life Sciences, 17(11): 1721-1732.
Reid et al., Methods in Enzymology, vol. LVIII, Academic Press, New York, 263-278 (1979).

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