Method for isolation of bovine low-molecular weight CR-binding s

Details Method for isolation of bovine low-molecular weight CR-binding s Method for isolation of bovine low-molecular weight CR-binding s

1996-10-11

1999-02-16

Tsang, Cecilia J.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

530350, C07K 1900

Patent

active

058721029

ABSTRACT:
A fully chromium loaded bovine low-molecular weight chromium-binding protein is isolated by a process that combines homogenization with supplementation of chromium content. Following homogenization with water, the homogenate is fractionated with ethanol, and the fractions obtained are subjected to serial chromatography (ion-exchange followed by size-exclusion chromatography) to obtain the biologically pure bovine LMWCr. This biologically pure material elutes from an HPLC column as essentially a single band, giving a high degree of purity. The LMWCr is useful as a dietary supplement, and for the treatment or prevention of a variety of chromium-related disease conditions.

REFERENCES:
Akiko Yamamoto et al., "Distribution and Chromium-Binding Capacity of A Low-Molecular-Weight, Chromium-Binding Substance In Mice", Journal Of Inorganic Biochemistry, vol. 22, pp. 91-102, 1984.
John B. Vincent, "Relationship Between Glucose Tolerance Factor And Low-Molecular-Weight Chromium-Binding Substance", Journal of Nutrition, vol. 124, pp. 117-118, 1994.
Akiko Yamamoto et al., "isolation Of A Biologically Active Low-Molecular-Mass Chromium Compound From Rabbit Liver", European Journal Of Biochemistry, vol. 165, pp. 627-631, 1987.
John B. Vincent, "Chromium: Biological Relevance", Encyclopedia Of Inorganic Chemistry, vol. 2, pp. 661-665, 1994.
Akiko Yamamoto et al., "Purification And Properties Of Biologically Active Chromium Complex From Bovine Colostrum", Journal of Nutrition, vol. 118, pp. 39-45, 1987.
Yamamoto, Caplus An #1989:550993 1989.
Yamamoto, Caplus An#1981:526830 1981.

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