Method for isolation and purification of enzyme-antibody conjuga

Details Method for isolation and purification of enzyme-antibody conjuga Method for isolation and purification of enzyme-antibody conjuga

1992-09-25

1993-11-30

Wityshyn, Michael G.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530350, 530813, 435188, 435975, 435177, C07K 320, C07K 1710, A23J 324

Patent

active

052666867

ABSTRACT:
A process is described for isolating an enzyme-antibody conjugate, wherein the enzyme is horseradish peroxidase or alkaline phosphatase, from an aqueous mixture of said conjugate and unconjugated enzyme. The process involves contacting the mixture with a water insoluble stationary phase having the Ni.sup.+2 ion chelated thereto and binding said conjugate to the stationary phase. The phase containing bound conjugate is then washed to remove unbound enzyme. Thereafter the conjugate is eluted from the stationary phase and recovered in a form substantially free of the unconjugated enzyme.

REFERENCES:
patent: 4504585 (1985-03-01), Reynolds
patent: 4551271 (1985-11-01), Hochuli
patent: 4650760 (1987-03-01), Chlebowski et al.
patent: 4877830 (1989-10-01), Dobeli et al.
patent: 5047324 (1991-09-01), Fredrickson
patent: 5116952 (1992-05-01), Martin et al.
patent: 5169936 (1992-12-01), Staples et al.

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