Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-09-20
1998-12-15
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
058494910
ABSTRACT:
Xylanase DNA is recovered from soil by PCR amplification using degenerate primers. Because of the complexity of the soil samples, it is likely that the recovered product will include more than one species of polynucleotide. These recovered copies may be cloned into a host organism to produce additional copies of each individual species prior to characterization by sequencing. Recovered DNA which is found to vary from known xylanases can be used in several ways to facilitate production of novel xylanases for industrial application. First, the recovered DNA, or probes corresponding to portions thereof, can be used as a probe to screen DNA libraries and recover intact xylanase genes including the unique regions of the recovered DNA. Second, the recovered DNA or polynucleotides corresponding to portions thereof, can be inserted into a known xylanase gene to produce a recombinant xylanase gene with the sequence variations of the recovered DNA.
REFERENCES:
patent: 5610048 (1997-03-01), Schulein et al.
Porteous et al. Current Microbiology 29: 301-307, 1994.
Tebbe et al. Applied and Environmental Microbiology 59: 2657-2665, 1993.
Steffan et al. Ann Rev, Microbiology 45: 137-61, 1991.
Smalla et al. J. of Applied Bscteriology 74: 78-85, 1993.
Porteous and Armstrong Current Microbiology 27: 115-118, 1993.
Porteous and ASrmstron Current Microbiology 22: 345-348, 1991.
Johnston and Aust Applied and Environmental Microbiology 60: 2350-2354, 1994.
Saddler, J.N., Bioconversion of Forest and Agricultural Plant Residues, CAB Int'l, Wallingford, England, pp. 1-11 (1993).
Wick, C.B., "Enzymology Advances Offer Economical and Environmentally Safe ways to Make Paper", Genetic Engineering News 14: 1 (1994).
McCubbin, N., "A Bleaching Revolution is in Progress", Pulp & Paper Canada 94: 12-16 (1994).
Tiedje, J.M., "Microbial Diversity: Of Value to Whom?", ASM News 60: 524-525 (1994).
Bergquist et al., "Hemicellulolytic Enzymes From Extremely Thermophilic Bacteria-Application of Molecular Genetics to Pulp Bleaching", poster presented at Society for Industrial Microbiology Meeting, Montreal, Canada, Jun. 1994.
Barns et al., "Remarkable archael diversity detected in a Yellowstone National Park hot spring environment", Proc. Nat'l Acad. Sci. (USA) 91: 1609-1613 (1994).
Holben et al., "DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community", Appl. Environ. Microbiol. 54: 703-711 (1988).
Patil et al., "PCR Amplification of an Escherichia coli gene using mixed primers contianing deoxyadensine at ambiguous positions in degenerate amino acid codons" Nucl. Acids Res. 18: 3080 (1990).
Don et al., "Touchdown PCR to circumvent spurious priming during gene amplification", Nucl. Acids Res. 19: 4008 (1991).
Roux, K.H., "Using Mismatched Primer-Template pairs in Touchdown PCR", BioTechniques 16: 812-814 (1994).
Matsumura et al, "DNA Shuffling Brightens Prospects for GFP" Nature Biotechnology 14:314 (1996).
Crameri et al., "Improved Green Fluorescent Protein by Molceular Evolution Using DNA Shuflfing", Nature Biotechnology 14: 315-319 (1996).
Munro et al., "A gene encoding a thermophilic alkaline serine proteinase from Thermus sp. strain Rt41A and its expression in Escherichia coli", Microbiology 141: 1731-1738 (1995).
Radomski Christopher C. A.
Seow Kah Tong
Warren R. Antony J.
Yap Wai Ho
Arthur Lisa B.
TerraGen Diversity Inc.
LandOfFree
Method for isolating xylanase gene sequences from soil DNA, comp does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for isolating xylanase gene sequences from soil DNA, comp, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for isolating xylanase gene sequences from soil DNA, comp will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1456507