Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-08-24
1998-09-08
Kight, John
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2541, 536 2542, 435270, 422 70, 422101, C07H 2100
Patent
active
058046844
ABSTRACT:
The invention features a method of isolating nucleic acid in a substantially purified form, including the steps of: a) contacting a biological sample which contains aggregated nucleic acid with a matrix comprising a solid hydrophilic organic polymer without an effective positive charge under conditions which permit the nucleic acid to bind to the matrix; and b) recovering nucleic acid from the matrix.
REFERENCES:
patent: 4160803 (1979-07-01), Potts
patent: 4902481 (1990-02-01), Clark et al.
patent: 4923978 (1990-05-01), McCormick
patent: 5063162 (1991-11-01), Kiefer
patent: 5108704 (1992-04-01), Bowers et al.
patent: 5187083 (1993-02-01), Mullis
patent: 5205989 (1993-04-01), Aysta
patent: 5217593 (1993-06-01), MacConnell
patent: 5219528 (1993-06-01), Clark
patent: 5234809 (1993-08-01), Boom et al.
patent: 5234824 (1993-08-01), Mullis
patent: 5264184 (1993-11-01), Aysta et al.
patent: 5346994 (1994-09-01), Chomczynski
patent: 5417923 (1995-05-01), Bojanic et al.
Freifelder, Physical Biochemistry, Second Edition, W. H. Freeman & Co., New York, NY, 1982, only copies of pp. 257-262 supplied by applicant.
Burtis et al., Tietz Textbook of Clinical Chemistry, Second Edition, W. B. Saunders Company, New York, NY, 1994, only copies of pp. 210-211 supplied by applicant.
Schleif & Wensink, Practical Methods in Molecular Biology, Springer-Verlag, New York, NY, 1981, only pp. 93-111 supplied.
"Proteinase K for Nucleic Acid Research," BMBiochemica, 1(5), 3 (Dec. 1984).
Beil et al., "Isolation of DNA from Fungal Mycelia and Sclerotia without Use of Density Gradient Ultracentrifugation," Analytical Biochem., 154, 21-25 (1986).
Beji et al., "A Rapid Chemical Procedure for Isolation and Purification of Chromosomal DNA from Gram-Negative Bacilli," Analytical Biochem., 162, 18-23 (1987).
Crane L. Eric
Kight John
The Theobald Smith Research Institute, Inc.
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