Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1992-12-16
1994-09-20
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 4351723, 4352402, 4352521, 4352522, 4352523, 43525231, 43525233, 4352525, 4352541, C12N 1500, C12N 500, C12N 112, C12N 114
Patent
active
053488725
ABSTRACT:
Disclosed is a method for isolating a mutant cell that secretes a desired compound at a level greater than that secreted by a starter cell from which the mutant cell is derived. The method includes contacting a plurality of auxotrophic feeder cells and auxotrophic starter cells together in the solid growth medium. The starter cells produce the desired compound required by the feeder cells and the feeder cells produce a metabolite required by the starter cells. These cells are cultured to produce a plurality of primary colonies, the largest of which include at least one of the mutant cells which overproduces the desired compound.
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Lin Edmund
Ray Bryan L.
Hill Jr. Robert J.
President and Fellows of Harvard College
Wang Gian P.
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