Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-08-23
1997-07-08
Walsh, Stephen G.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 5, 435 691, 4351721, 4352351, 435239, 4352523, 43525233, 43525411, 435373, C12N 500, C12P 2106
Patent
active
056460309
ABSTRACT:
Disclosed is a method for isolating a mutant cell that excretes a desired compound. The method includes culturing a plurality of auxotrophic pretreated starter cells and auxotrophic feeder cells in the presence of a reversibly noninfective, modified lambdoid bacteriophage. If the treated starter cell produces the desired compound, the bacteriophage will be rendered infective and infect the feeder cell. The feeder cell, in turn, will excrete a metabolite required by the starter cell and the starter cell will excrete a metabolite required by the feeder cell, enabling the cells to cross-feed, grow, and produce a colony containing a starter cell which produces the desired compound.
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Crea Roberto
Lin Edmund C. C.
Ray Bryan L.
President and Fellows of Harvard College
Sorensen Kenneth A.
Walsh Stephen G.
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