Method for isolating MB creatine kinase

Details Method for isolating MB creatine kinase Method for isolating MB creatine kinase

1978-02-15

1980-04-29

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435815, C12Q 150

Patent

active

042006910

ABSTRACT:
Creatine kinase MB isoenzyme can be separated from a mixture of creatine kinase isoenzymes by ion exchange chromatography. Use of a mixture of cation and anion exchange resin in this method permits elution of the MB isoenzyme from the mixed resin while the other isoenzymes are wholely or partially retained. In a preferred embodiment the mixed resin is a mixture of a weakly basic anion exchange resin and a weakly acidic cation exchange resin.

REFERENCES:
patent: 4049496 (1977-09-01), Henry
patent: 4105499 (1978-08-01), Kiyasu
Calmon et al. editors, Ion Exchangers in Organic and Biochemistry, 1957, pp. 380, 492, 630.
Takahashi et al., Clinica Chimica Acta, vol. 38, pp. 285-290 (1972).
Clinical Chemistry, vol. 20, No. 1, pp. 36-40 (1974).
Clinical Chemistry, vol. 21, No. 3, pp. 381-386, pp. 392-397 (1975).
Clinical Chemistry, vol. 21, No. 7, pp. 844-849 (1975)._
Clinical Chemistry, vol. 21, No. 8, pp. 1088-1092 (1975)._
Clinical Chemistry, vol. 22, No. 1, pp. 92-97 (1976)._
Clinical Chemistry, vol, 22, No. 4, pp. 552-554 (1976).

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