Method for isolating inhibitors of protease activity

Details Method for isolating inhibitors of protease activity Method for isolating inhibitors of protease activity

1998-03-27

2000-01-25

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 23, 435 4, 562125, C12Q 134, C12Q 137, C12Q 100

Patent

active

060177233

ABSTRACT:
The present invention provides an assay for detecting and quantifying protease that is sensitive, rapid, and capable of screening numerous samples of small volume. Also provided by the present invention is a method for diagnosing disorders associated with connective tissue destruction in a subject by detecting and quantifying a particular protease in a biological sample. Further provided by the present invention is a method of detecting inhibitors of the activity of a protease by detecting primary amines that are exposed by the enzymatic degradation of a protease substrate.

REFERENCES:
patent: 5073487 (1991-12-01), Lloyd
patent: 5338663 (1994-08-01), Potter et al.
patent: 5545640 (1996-08-01), Beaulieu et al.
Instructions for QuantiCleave.TM. Protease Assay Kit II by Pierce, 3747 N. Meridian Road, P.O. Box 117, Rockford, IL 61105.
Rao et al; Analytical Biochemistry; vol. 250, pp. 222-227, (1997).

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