Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1993-05-03
1994-10-25
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
5303871, 5303901, 530861, 530862, 530863, A61K 39395
Patent
active
053590384
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a method for isolating the immunoglobulin compounds, namely, IgAs, IgMs, IgD, IgG and its subclasses 1; 2; 3 and 4 in human and animal feces, and more particularly, to such method that is used in the diagnosis and treatment of allergies and diseases associated with deficiencies in the immulogical system of patients.
BACKGROUND ART
Applicant believes that the closest reference corresponds to a seminar that took place in San Francisco and sponsored by the American College of Allergy & Immulogy (47th Annual Meeting) in November of 1990 (lecturers: J. E. Postley, M. D. and J. Einbinder, PhD) wherein it was discussed that IgAs can be obtained from the saliva to make certain determinations concerning the presence of antigens/antibodies. The method disclosed, however, required the production of approximately 30 cc of saliva from a patient in order to obtain IgAs in the micro-grams range. The unreliability of using any conventional diagnosis method from these minimal quantities of IgAs is quite apparent. Also, the undesirability of the method from the patient's standpoint is obvious.
To determine the presence of antigens and/or antibodies many conventional methods have been used in the past. Most of them, using an enzyme conjugated with another chemical substance to detect the presence, and with some methods, approximate the quantity of the antigens and antibodies. Typically, these methods are used in conjunction with blood drawn from the patient and the immunoglobin classes tested are the IgE and IgG4. However, IgAs is not present in the blood drawn from an individual in appreciable quantities since it is mainly created by the mucous, such as the intestines' mucous membranes, and being eventually stored in the feces. The information carried in general immunity IgE and IgG4 is not as relevant for diagnostic purposes as the information included in the local immunity IgAs. The conventional methods include those that use conjugated enzymes, such as, ELISA, RIA, radio immuno assay, immunoofluorescent methods, dots/disks, strips methods, etc. None of these methods are directed towards the use of IgAs since it is not present in the blood drawn from the patient in appreciable quantifies.
None of the methods presently used for detection of the presence of antigens and/or antibodies utilize IgMs because it is not found in the blood in sufficient amounts. However, when used with IgAs it provides valuable information in the diagnostis of diseases caused by bacterias, viruses and certain tumural lesion on the digestive tract.
SUMMARY OF THE INVENTION
It is one of the main objects of the present invention to provide a method for detecting, identifying qualitatively and quantitatively the presence of antigens and antibodies in a person or animal through the isolation of the immunoglobulin compounds.
It is another object of this invention to provide a method that is reliable and does not present inconveniences to the user.
It is still another object of the present invention to provide a method that can be readily used.
It is yet another object of this invention to provide such a method that is inexpensive to utilize.
It is yet another object of this present invention to provide such a device that is inexpensive to manufacture and maintain while retaining its effectiveness.
Further objects of the invention will be brought out in the following part of the specification, wherein detailed description is for the purpose of fully disclosing the invention without placing limitations thereon.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT
The preferred manner of practicing the present invention will be described below. The inventor has obtained excellent results, compared to the other conventional methods. The key is to isolate a sufficiently large amount of the immunoglobulin compounds in order to accurately determine the presence of antigens and/or antibodies. The different immunoglobulin compounds, namely, IgAs, IgMs, IgD, IgG, IgG.sub.1, IgG.sub.2, IgG.sub.3 and IgG.sub.4,
REFERENCES:
patent: 5155213 (1992-10-01), Padron
Sanchelima J.
Schain Howard E.
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