Method for isolating high purity peroxidase

Details Method for isolating high purity peroxidase Method for isolating high purity peroxidase

1975-04-21

1976-03-30

Shapiro, Lionel M.

Textiles: fiber preparation

C07G 7022

Patent

active

039473244

ABSTRACT:
The present invention encompasses a method for isolating peroxidase enzyme from plant tissue containing peroxidase. The invention has as its critical step treating an aqueous extract of said plant tissue having the pH adjusted to 6 - 9 with at least 2.7 .times. 10.sup.-.sup.3 moles per liter of zinc ion. Unexpectedly at this pH the zinc ion selectively precipitates contaminating impurities from the extract and thereby provides a substantially purified peroxidase solution from which peroxidase is isolated by a variety of conventional techniques. Thus the critical step of the present invention is used in conjunction with salt fractionation, solvent fractionation, dialysis, reverse osmosis, electrophoresis, column chromatography and other techniques for purifying protein. The peroxidase isolated by methods of the present invention is useful in analytical diagnostic kits and other applications in enzyme and substrate assays in conjunction with a chromogenic donor in assaying systems producing hydrogen peroxide.

REFERENCES:
methods in Enzymology, Vol. II, pp. 801-813 (1955).
Chemical Abstracts, Vol. 69, 74046v (1968).

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