Method for isolating DNA

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S025400, C536S025410, C536S025420

Reexamination Certificate

active

06969603

ABSTRACT:
Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier. The method also includes separating the DNA-bound carrier from other components. The method further includes dissociating the bound DNA from the DNA-binding carrier. The method still further includes recovering dissociated DNA.

REFERENCES:
patent: 4833239 (1989-05-01), DeBonville et al.
patent: 5010183 (1991-04-01), Macfarlane
patent: 5204382 (1993-04-01), Wallace et al.
patent: 5234809 (1993-08-01), Boom et al.
patent: 5342931 (1994-08-01), Woodard et al.
patent: 5520899 (1996-05-01), Woodard et al.
patent: 5547677 (1996-08-01), Wright
patent: 5596092 (1997-01-01), Schneider
patent: 5660984 (1997-08-01), Davis et al.
patent: 5948826 (1999-09-01), Terada et al.
patent: 5990301 (1999-11-01), Colpan et al.
patent: 6342387 (2002-01-01), Hayashizaki et al.
patent: 4-360686 (1992-12-01), None
patent: 7-250681 (1995-10-01), None
patent: 8-23976 (1996-01-01), None
Naktinis et al., “Two Simple Methods for Isolation of DNA from Various Sources Using Cetavlon”,Biokhimiya, 42(10), pp. 1783-1790 (1977), —English translation only.
Ellington. 1993. Purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis. pp. 2.12.1-2.12.5 in Ausubel et al., eds. Current Protocols in Molecular Biology. John Wiley & Sons, Inc.
Reichardt et al. 1994. Preparation of genomic DNA from plant tissue. pp. 2.3.1-2.3.7 in Ausubel et al.., eds. Current Protocols in Molecular Biology. John Wiley & Sons, Inc.
Budelier. 1993. Purification of DNA by anion-exchange chromatography. pp. 2.14.1-2.14.8. in Ausubel et al., eds. Current Protocols in Molecular Biology. John Wiley & Sons, Inc.
Richards. 1993. Separation of double-and single-stranded nucleic acids using hydroxylapatite chromatography. pp. 2.13.1-2.13.3. in Ausubel et al., eds. Current Protocols in Molecular Biology. John Wiley & Sons, Inc.
Joyce Goudy Guenther, “A New, Versatile Solid Phase Matrix for Rapid, Selective Extraction and Purification of DNA or RNA form Experimental Mixtures”,BioTechniques, vol. 2, No. 5, 1984, pp. 320-325, XP002112450.
Masayoshi Itoh et al., “Simple and Rapid Preparation of Plasmid Template by a Filtration Method Using Microtiter Filter Plats”, Nucleic Acids Research, vol. 25, No. 6, 1997, pp. 1315-1316, XP002216401.
English Language Abstract of JP 4-360686 Dec. 4, 1992.
English Language Abstract of JP 8-23976 Jan. 30, 1996.
English Language Abstract of JP 7-250681 Oct. 3, 1995.
Schneider, “Simplified Isolation and Quantitation of Cytoplasmic DNA from Rat Liver”,Analytical Biochemistry, 103, pp. 413-418 (1980).
Tong et al., “Solid-Phase Method for the Purification of DNA Sequencing Reactions”,Anal. Chem., 64, pp. 2672-2677 (1992).
Boom et al., “Rapid and Simple Method for Purification of Nucleic Acids”Journal of Clinical Microbiology, vol. 28, No. 3, pp. 495-503 (Mar. 1990).
English Translation of Naktinis et al., “Two Simple Methods for Isolation of DNA from Various Sources Using Cetavlon”,Biokhimiya, 42(10), pp. 1783-1790 (1977).
Hjelmeland. 1990. Solubilization of native membrane proteins. pp. 253 and 257. In Deutscher, et. Guide to protein purification. Academic Press, Inc..
Eisenberg et al., 1990. Purification of DNA-binding proteins by site-specific DNA affinity chromatography, pp. 521, 524, 525. In Deutscher, et. Guide to protein purification. Academic Press, Inc..
Sambrook et al., “Molecular Cloning”, Second Edition, pp. E.30-E33 (1989).
Rogers et al., “Extraction of DNA from Milligram Amounts of Fresh, Herbarium amd Mummified Plant Tissues”,Plant. Mol. Biol., 5:69-76 (1985).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for isolating DNA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for isolating DNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for isolating DNA will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3499918

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.